Infections, Infertility, and Assisted Reproduction

Portada
Cambridge University Press, 2004 M12 9
ART treatment is vulnerable to the hazard of potential infection from many different sources: patients, samples, staff and the environment. Culture of gametes and embryos in vitro provides multiple targets for transmission of potential infection, including the developing embryo, neighbouring gametes and embryos, the couple undergoing treatment and other couples being treated during the same period. This unique situation, with multifaceted opportunities for microbial growth and transmission, makes infection and contamination control absolutely crucial in the practice of assisted reproduction, and in the laboratory in particular. Originally published in 2004, this practical book provides a basic overview of microbiology in the context of ART, providing a guide to infections in reproductive medicine. The relevant facets of the complex and vast field of microbiology are condensed and focused, highlighting information that is crucial for safe practice in both clinical and laboratory aspects of ART.
 

Contenido

Introduction
3
History of assisted reproduction
7
In vitro fertilization
8
Assisted reproductive technology ART and microbiology
10
Overview of microbiology
11
References
14
Further reading
15
glossary of terms
16
Further reading
165
antiparastic agents
166
FURTHER READING
173
Infections in reproductive medicine
175
Genital ulcer diseases
177
Syphilis
185
Chancroid
190
Lymphogranuloma venereum LGV
192

Bacteriology
21
Bacterial growth
26
Bacterial classification and identification
27
Major groups of organisms
38
Grampositive cocci that are catalase positive
42
Grampositive cocci that are catalasenegative
43
Grampositive bacilli that are nonbranching and catalase positive
44
Grampositive bacilli that are nonbranching and catalase negative
46
Grampositive bacilli that are branching or partially acidfast
47
Mycobacteria and bacteria with unusual growth requirements
50
Normal flora in humans
54
Further reading
61
Media used for isolation of bacteria
62
Biochemical tests for identification of bacteria
67
Antibacterial agents
85
Further reading For Appendix 23
89
Mycology moulds and yeasts
90
Classes of fungi
91
Zygomycetes
92
Clinical classification of fungi
94
Contaminants
98
Laboratory identification of fungi
99
Microscopic examination for fungal structures
100
antifungal agents
102
Virology
105
Host range and specificity
106
Lytic growth
107
Singlestranded DNA
109
Laboratory diagnosis of viral disease
110
Serologic diagnosis
111
Doublestranded DNA viruses
112
Hepatitis viruses
115
Retroviruses
116
Human oncornaviruses
117
Further reading
118
antiviral agents
119
Prions
122
Replication
124
Clinical presentation
126
Diagnosis
127
References
128
Further reading
129
Parasitology
131
protozoa
134
Tissue amoeba
135
Sarcomastigophora flagellates
136
Ciliophora ciliates
139
Plasmodium
140
Piroplasma
141
Toxoplasma gondii
142
Isospora belli
143
Microsporidia
145
Enterobius
146
Ascaris
147
Hookworm
148
Trichinella spiralis
149
Dracunculus medinensis
150
Visceral larval migrans
151
Filarial parasites
152
Loa loa
153
Mansonella species
154
Hymenolepis diminuta
155
Dipliyllobothrium lactum
156
Dipylidium caninum zoonotic cestode infection
157
Echinococcus granulosus zoonotic cestode infection
158
Trematodes flukes
159
Fasciolopsis buski
160
Clonorchis sinensis
161
Schistosoma species the blood flukes
162
Schistosome zoonosis
163
Arachnida
164
Granuloma inguinale Donovanosis
193
References
195
Vaginitis syndromes
199
Trichomonas vaginalis
200
Yeast vaginitis
203
Bacterial vaginosis
207
Vaginal colonization with Group B
209
Further reading
212
Genital human papillomavirus HPV infections
215
Further reading
219
Urethritis and cervicitis syndromes
220
Gonorrheal disease
221
Chlamydial disease
228
Chlamydia trachomatis
229
Laboratory diagnosis
231
Genital mollicutes
234
Clinical presentation
235
Clinical diagnosis
236
Molecular diagnosis
238
REFERENCES
239
REFERENCES
241
Pathology of the upper genitourinary tract
243
Orchitis
244
Female upper GU infections
245
Oophoritis
246
Pelvic inflammatory disease PID
247
Clinical diagnosis
249
Genital tuberculosis
250
Laboratory diagnosis
255
Further reading
259
Cytomegalovirus and bloodborne viruses
262
Hepatitis B virus HBV
270
Hepatitis C virus HCV
275
Hepatitis D virus HDV
278
HIV and AIDS
281
Human Tlymphotrophic viruses HTLV
290
HTLVI
291
References
293
specimen culture by body site
299
Infection and contamination control in the ART laboratory
305
Sterilization methods
316
Chemical methods of sterilization
318
Disinfection and decontamination
320
Air quality classification of cleanrooms and biological safety cabinets
321
Biological safety cabinets BSCs
322
Microbiological testing and contamination
325
Laboratory cleaning schedules
327
References
330
Further reading
331
Handling infectious agents in the ART laboratory
332
Biosafety levels
333
Biosafety for ART
334
Treatment of HBV seropositive couples
336
Treatment of HIV seropositive couples
337
Semen washing procedures for HBVHCVHIV serodiscordant couples
339
Virus decontamination
340
HBV prophylaxis
341
Air transport of biohazardous materials
342
Useful addresses for air transport of hazardous materials
349
general laboratory safety
350
Further reading
351
Prevention patient screening and the use of donor gametes
353
Recruitment of donors
355
Screening
356
Procedures and technical aspects
357
Treatment evaluation
358
ovarian and testicular tissue
361
References
362
Further reading
363
Index
365
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Página 3 - I took out of my mouth, after ridding it of air bubbles (lest the bubbles should make any motion in the spittle): and I then most always saw, with great wonder, that in the said matter there were many very little living animalcules, very prettily a-moving.
Página 3 - A): these had a very strong and swift motion, and shot through the water (or spittle) like a pike does through the water. These were most always few in number. The second sort had the shape of fig. B. (fig. 4 B). These oft-times spun round like a top, and every now and then took a course like that shown between C and D: (fig.
Página 3 - ... spittle out of his mouth and examined it; but I could find in it nought but what I had found in my own and other people's. I also took some of the matter that was lodged between and against his teeth, and mixing it with his own spit, and also with fair water (in which there were no animalcules), I found an unbelievably great company of living animalcules, a-swimming more nimbly than any I had ever seen up to this time. The biggest sort (whereof there were a great plenty) bent their body into...

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